Dynamic and highly motivated Industrial Biotechnologist with expertise in producing and purifying biological products. Passionate about driving innovation in cutting-edge research environments with a strong commitment to advancing biotechnological solutions.
About Me
I am, Abdul Basit
Looking for a position as a Research Associate/junior bioprocess engineer a production technologist, or process developer in R&D or for the production of food, vaccines, enzymes, and other industrial bioproducts involving either pharmaceutical or an industrial biotech sector.
What is my skill level ?
As a biotechnologist with a focus on up-streaming and down-streaming, My skill level can be described as proficient. I have a solid understanding of the techniques and processes involved in both up-streaming and down-streaming, and can apply this knowledge in a laboratory setting. I am comfortable with the use of various equipment and instrumentation, and can troubleshoot and solve problems that may arise during experiments. Additionally, I have experience with data analysis and interpretation and can effectively communicate my findings. Overall, I am confident in my abilities and continue to strive for growth and improvement in the field of biotechnology.
Education
I am really happy that I am successful in my educational journey and looking forward to more achievements.
Masters in Pharmaceutical & Industrial Biotechnology
2021 - 2024 Martin Luther University Halle (Saale)Bachelors (Hons) in Biotechnology
2016 - 2020 University of Sargodha, Punjab PakistanSkills
Highly able to combine current academic and theoretical knowledge with practical experience to provide solutions-oriented service.
Molecular Biotechnology (PCR)
Up-streaming
Fermentation technology (Sartorius 5L bio-stat stainless steel)
Down-streaming
Chromatography (ÄKTA prime plus) (HPLC)
Ultra-filtration
PrimeView 5.0
IKA fermenters (2L and 5L) & IKA Software
DASWARE (Eppendorf) & DASGIP System
Analytical Enzymatic assays
Basics of Berkeley Medona software
Experience
Experience in working for optimizing the upstream process design of experiment (DoE) and also have some basic knowledge of Downstream Processing in finding protein concentration and other analysis parameters.
Chemical Technical AssistantQ
06/2024 - present INSEMPRA GmbH IZB Munich- ⋄ Worked as a CTA in the bio-processing department to produce oil (C16:1,
C18:1) using wild type & genetically engineered yeast species (Wickerhamomyces & Rhodotorula).
⋄ Worked on different bioreactor systems (DSGIP & IKA 2L & 10L fermenters).
⋄ Worked on different projects involved to optimize media, enhance biomass production, and increasing titers.
⋄ Worked on the SPRIND project, which not only aimed to increase the production of oil but also contributed
to circular economy through sustainability.
⋄ Worked to increase 2 PE (Phenyethanol) production using a column with different adsorption material on a
lab scale.Gained experience in daily laboratory tests.
.
Resaerch Assistant and Master’s Thesis Student
05/2023 - 05/2024 Biozentrum, Weinberg Campus Halle (Saale)- ⋄ Topic: Down-scaling and Intensification of Fed-batch fermentation production of Asparaginase B with E.coli.
⋄ production using E.Coli BL21pET11a-ansB.
⋄ Downscaling from 20 L to 5 L fermenter volume.
⋄ By increasing in Amount of Biomass, our protein concentration was increased from 8 g/L to 10 g/L.
⋄ Assisted professor and supervised Master students in upstream processing (Lab task) and developed SOP and
laboratory protocols to ensure compliance with quality standards.
Projects
- ⋄ Continuous fermentation production of levansucrase with Bacillus Megaterium in Sartorius 5L bio-stat stainless steel. The aim was to increase
biomass production.
⋄ Fed-batch fermentation production of epsilon-Caprolactone using whole-cell biocatalysis in 2L Flat-panel bioreactor using Green E.Coli photosynthetic
cyanobacteria Synechocystis PCC6803.
⋄ Down-streaming of Asparaginase B from E.Coli BL21 pET11a-AnsB using Ion exchange chromatography. LAsparaginase is successfully produced with a mean
specific activity of 84.77 U / mg with an enrichment factor of 8.2.
⋄ Softwares: Berkeley Medona, DSWARE (Eppendorf), IKA Software, OpenChrom (Lablicate) and PrimeView 5.0
Contact Us
You can contact me through different platform :
Phone
Address
- Knorrstrasse 28, 80807, Munich, Germany